Antibacterial activity of a plant extract and its potential for disinfecting gutta-percha cones.
نویسندگان
چکیده
This study evaluated the antibacterial activity of Rosmarinus officinalis extract and its potential for disinfecting guttapercha (GP) cones. In the first experiment, a hydro-alcoholic extract of Rosmarinus officinalis (leaves) in a dilution ratio of 10% m/v was tested against Enterococcus faecalis by using the disk diffusion method. Positive and negative controls were 70% cereal alcohol and antibiotics, respectively. The procedures were performed in triplicate, and the diameters of the zones of growth inhibition were measured with a caliper after 24 h at 37 degrees C. In the second experiment, the disinfection procedures were evaluated on GP cones artificially contaminated with Enterococcus faecalis. The R. officinalis extract was compared with 2% chlorhexidine digluconate and 2.5% sodium hypochlorite, using a direct exposure test (5 min treatment). Sterilized and non-disinfected cones were used as negative and positive controls, respectively. After 24 h of incubation, bacterial counts were taken. For both experiments, the data were statistically analyzed by Kruskall-Wallis and Tukey's tests (p < 0.05). The plant extract produced zones of inhibition comparable to those of tested antibiotics. Significant GP cone disinfection was verified with all disinfectant solutions, with no significant difference between them. R. officinalis extract showed bactericidal effect on Enterococcus faecalis and capacity to disinfect GP cones contamined with it.
منابع مشابه
To Evaluate the Antimicrobial Activity of Herbal Extracts and their Efficacy in Disinfecting Gutta Percha Cones Before Obturation- an in Vitro Study
Context: Gutta percha are known to be contaminated during storage and intra operative manipulation. Chair side disinfection of these points is important and critical for success of endodontic therapy. Gutta percha being heat labile, chemical disinfection is the only viable alternative which takes longer time and may leave a residue. Herbal gels are known to have antimicrobial , antiseptic prope...
متن کاملDisinfection of gutta-percha cones with chlorhexidine and sodium hypochlorite.
OBJECTIVES To evaluate the effectiveness of chlorhexidine (CHX) or sodium hypochlorite (NaOCl) in disinfecting gutta-percha cones, to verify contamination of gutta-percha cones in their boxes, and to identify microorganisms after intentional contamination by handling cones. STUDY DESIGN Gutta-percha cones contaminated in vitro with several microorganisms were left in contact with tested disin...
متن کاملEvaluation of Disinfection of Gutta‐ Percha Cones Using Different Chemical Solutions
Background: Disinfection and sterilization of obturating materials in the root canal is necessary for the successful root canal therapy. Various disinfectants have been used to sterilize and disinfect gutta‐ percha including sodium hypochlorite (NaOCl), chlorohexidine (CHx), etc. . Effectiveness of peracetic acid over NaOCl is documented in literature, but no study to date has shown the effect ...
متن کاملEvaluation of antimicrobial activity and efficacy of herbal oils and extracts in disinfection of gutta percha cones before obturation
Objectives Literature has shown that micro-organisms contaminate gutta percha (GP) during storage and manipulation. Till date herbal extracts are not explored as an alternative medicament for pre-operative chairside disinfection of GP cones. The purpose of our study was to evaluate the antimicrobial activity and efficacy of lemon grass oil (LG), basil oil (BO), and obicure tea extract (OT) in d...
متن کاملمقایسه آلودگی زدایی مخروطهای گوتاپرکا با سه نوع محلول ضدعفونی کننده در مدت زمان یک دقیقه
Background and Aim: Care must be taken during root canal therapy to prevent contamination of filling materials and avoid root canal contamination. Gutta-percha cones are now widely used to fill root canals. However they are not resistant to conventional sterilization processes in moist or dry heat. To keep the aseptic chain, gutta-percha cones require rapid chair side decontamination before use...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Acta odontologica latinoamericana : AOL
دوره 25 1 شماره
صفحات -
تاریخ انتشار 2012